붉은자루동충하초의 항염물질의 생리활성확인(3)

라. CPME의 primary peritoneal macrophge에서의 iNOS COX-2저해효과

cell line인 Raw 264.7에서는 iNOS와 COX-2의 발현을 억제하였기에 primary cell culture를 통한 세포에서도 같은 결과가 형성되는지를 알아본 결과 LPS에의해서 유도된 iNOS. COX-2의 발현을 CPME가 억제시키는 것을 알 수가 있었다(Fig 24).

 

Fig. 24. Effects of the CPME on iNOS and COX-2 ｅxpression in primary peritoneal macrophages. Primary macrophages isolated from mouse peritoneal cavity were precultured overnight and treated with LPS (1 ㎍/ml) in the presence of the CPME for 18 h. (A) Nitrite was measured in culture media by the Griess reaction. Data are shown the mean ± SD (n=4). ＊, p＜0.05; ＊＊, p＜0.01 versus LPS alone. (B) The levels of iNOS and COX-2 were measured by Western blot analysis as described in Figs 1 and 2.