붉은자루동충하초의 항염물질의 생리활성확인(5)

바. in vivo에서의 CPME에 의해서 NO, PGE2, IL-1β, 그리고 TNF-α 생성저해 효과

NO, PGE2, TNF-α 그리고 IL-1β는 endotoximia를 포함한 여러 염증질병에 관련되어져 있다. 항염증약은 LPS로 복용된 동물모델에서 이 염증 조절자들을 억제하는 것을 알 수가 있다. 이에 LPS로 처리된 쥐에서 과연 이 cytokine이 억제되는지를 확인한 결과 모두 CPME의 농도에 따라서 이 cytokine의 생성이 감소되었다(Fig 26).

 

Fig. 26 Effect of the CPME on production of NO, PGE2 , TNF-α, IL-1β by LPS-injected mice.

Mice were i.p. injected twice with the CPME or saline with 24h interval. At 12 h following the second injection, LPS or saline was i.p. injected. Twelve h later, whole blood was collected by cardiac puncture and plasma prepared by centrifugation. The plasma levels of nitrite plus nitrate(NOx) were measured using a nitrate assay kit. The plasma levels of PGE2, TNF-α, IL-1β were measured using ELISA kits. All data represent the mean ± SD from the data of 7 animals per group. ＊, p＜0.01 and ＊＊, p＜0.01 versus control and LPS alone, respectively.